7KTG

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 50 mM Mg2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.070, 68.523, 110.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.067 - 1.445
R-factor	0.159
Rwork	0.158
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.445	3.940	1.450
Rmerge	0.127	0.112	0.721
Rmeas	0.140	0.124	0.818
Rpim	0.058	0.052	0.377
Number of reflections	81520	4300	3954
<I/σ(I)>	10.8
Completeness [%]	99.0	97.1	97.3
Redundancy	5.6	5.5	4.2
CC(1/2)		0.976	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000