7KTD

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 10 mM Mn2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-15
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.316, 62.420, 119.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.375 - 1.551
R-factor	0.1609
Rwork	0.160
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.078	0.081	0.634
Rmeas	0.085	0.088	0.701
Rpim	0.032	0.035	0.293
Total number of observations	466114
Number of reflections	65749	3477	3197
<I/σ(I)>	12.1
Completeness [%]	99.8	97.6	99.1
Redundancy	7.1	6.5	5.4
CC(1/2)		0.985	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000