7KTB

DNA Polymerase Mu, 8-oxodGTP:Ct Reaction State Ternary Complex, 10 mM Mn2+ (40min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.278, 62.319, 119.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.038 - 1.576
R-factor	0.1592
Rwork	0.158
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.576	4.290	1.580
Rmerge	0.056	0.035	0.982
Rmeas	0.061	0.039
Rpim	0.026	0.017	0.455
Number of reflections	119332	3376	3077
<I/σ(I)>	13
Completeness [%]	100.0	99.6	100
Redundancy	5.9	5.5	5.6
CC(1/2)		0.997	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000