7KT5

DNA Polymerase Mu, 8-oxodGTP:At Product State Ternary Complex, 10 mM Mn2+ (120min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.996, 68.701, 110.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.932 - 1.460
R-factor	0.1628
Rwork	0.162
R-free	0.18250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.490
High resolution limit [Å]	1.460	3.960	1.460
Rmerge	0.070	0.061	0.763
Rmeas	0.078	0.069	0.855
Rpim	0.034	0.030	0.380
Total number of observations	412456
Number of reflections	78861	4069	3778
<I/σ(I)>	13.5
Completeness [%]	99.0	95	97.5
Redundancy	5.2	5.1	4.6
CC(1/2)		0.992	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000