7KT2

DNA Polymerase Mu, dGTP:At Product State Ternary Complex, 50 mM Mn2+ (225min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.893, 68.866, 110.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.958 - 1.499
R-factor	0.1689
Rwork	0.168
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.010
RMSD bond angle	1.107
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.499	4.070	1.500
Rmerge	0.057	0.040	0.805
Rmeas	0.063	0.044	0.886
Rpim	0.025	0.018	0.362
Number of reflections	73665	3959	3495
<I/σ(I)>	11.1
Completeness [%]	99.7	99.6	97
Redundancy	6.2	5.9	5.5
CC(1/2)		0.996	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG 4000