7KT0

DNA Polymerase Mu, dGTP:At Ground State Ternary Complex, 50 mM Mg2+ (60min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.918, 68.568, 110.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.284 - 1.360
R-factor	0.1622
Rwork	0.161
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.380
High resolution limit [Å]	1.360	3.690	1.360
Rmerge	0.089	0.092	0.788
Rmeas	0.097	0.100	0.889
Rpim	0.036	0.038	0.400
Total number of observations	669543
Number of reflections	97735	5212	4707
<I/σ(I)>	12.2
Completeness [%]	99.8	99.5	98.1
Redundancy	6.9	6.5	4.6
CC(1/2)		0.982	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG 4000