7KSV

DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mn2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.002, 68.825, 110.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.946 - 1.640
R-factor	0.1636
Rwork	0.162
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.670
High resolution limit [Å]	1.638	4.450	1.640
Rmerge	0.063	0.030	1.032
Rmeas	0.069	0.034	1.140
Rpim	0.028	0.014	0.475
Number of reflections	56764	3068	2834
<I/σ(I)>	11.4
Completeness [%]	99.8	99.6	99.6
Redundancy	5.7	5.4	5.2
CC(1/2)		0.999	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000