7KSP
Crystal structure of hSAMD9_DBD with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.796, 59.022, 88.383 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2157 |
| Rwork | 0.208 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.218 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.054 | 0.499 |
| Number of reflections | 16022 | 1976 |
| <I/σ(I)> | 6.7 | 1 |
| Completeness [%] | 89.3 | 59.9 |
| Redundancy | 1.7 | 1.5 |
| CC(1/2) | 0.996 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium acetate, 20mM magnesium chloride, 20% PEG3350 |
