7KRV
Stimulating state of disulfide-bridged Hsp70 DnaK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.698, 98.698, 382.605 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.350 - 1.920 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kru |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.350 | 49.350 | 1.960 |
| High resolution limit [Å] | 1.920 | 10.180 | 1.920 |
| Rmerge | 0.200 | 0.037 | 4.255 |
| Rmeas | 0.209 | 0.039 | 4.474 |
| Rpim | 0.062 | 0.012 | 1.358 |
| Total number of observations | 937591 | 6833 | 43701 |
| Number of reflections | 84808 | 717 | 4240 |
| <I/σ(I)> | 7.7 | 34.1 | 0.5 |
| Completeness [%] | 99.8 | 99.3 | 96.3 |
| Redundancy | 11.1 | 9.5 | 10.3 |
| CC(1/2) | 0.998 | 1.000 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.04 M HEPES pH 7.5, 25.5% PEG 4000, 15% glycerol, 0.17 M ammonium sulfate, 0.01 M citric acid, 3% PEG 6000 |
