7KRM
Putative FabG bound to NADH from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | H 3 |
| Unit cell lengths | 189.872, 189.872, 71.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.910 - 1.900 |
| R-factor | 0.1516 |
| Rwork | 0.150 |
| R-free | 0.17390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7krk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.435 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.910 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 76202 | 4540 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.997 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M sodium acetate trihydrate, pH 4.6, 2 M sodium formate |
