7KQY
Crystal Structure and Characterization of Human Heavy-Chain only Antibodies reveals a novel, stable dimeric structure similar to Monoclonal Antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.619, 73.418, 140.408 |
| Unit cell angles | 90.00, 125.71, 90.00 |
Refinement procedure
| Resolution | 114.009 - 2.913 |
| R-factor | 0.2097 |
| Rwork | 0.207 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vsi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.850 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (1.14_3260) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.100 | 3.020 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Number of reflections | 22757 | 131 |
| <I/σ(I)> | 4.85 | |
| Completeness [%] | 75.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.979 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 303.15 | 0.2 M Ammonium Sulfate, 0.1M Bis-Tris pH 5.5, 25 % w/v PEG 3350 |
