7KQM

Binary complex of TERT (telomerase reverse transcriptase) with RNA/telomeric DNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	96.350, 96.350, 368.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.840 - 2.730
R-factor	0.1943
Rwork	0.193
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyl
RMSD bond length	0.006
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.730	2.730
Number of reflections	28107	2020
<I/σ(I)>	19.1	2.02
Completeness [%]	100.0
Redundancy	20.9
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.18 M tri-ammonium citrate, 20 % PEG 3350