7KQI

Antibodies that engage the hemagglutinin receptor-binding site of influenza B viruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	128.100, 128.100, 235.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.610 - 4.200
R-factor	0.3769
Rwork	0.372
R-free	0.42030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqj 4fql
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.610	50.000	4.500
High resolution limit [Å]	4.200	40.000	4.200
Rmerge	0.740	0.094	6.648
Rmeas	0.760	0.098	6.800
Total number of observations	182579
Number of reflections	8864	7	1597
<I/σ(I)>	3.51	15.58	0.61
Completeness [%]	99.8	100	100
Redundancy	20.598	9.429	22.595
CC(1/2)	0.992	0.999	0.240

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200 mM sodium citrate pH 7.0, 20% (wt/vol) PEG 3350