7KQH

Antibodies that engage the hemagglutinin receptor-binding site of influenza B viruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	132.660, 132.660, 93.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	114.890 - 3.500
R-factor	0.2546
Rwork	0.252
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqj 4fql
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	114.890	114.890	3.600
High resolution limit [Å]	3.500	50.000	3.500
Rmerge	0.253	0.070	6.602
Rmeas	0.276	0.076	7.218
Total number of observations	145358
Number of reflections	23115	7	1897
<I/σ(I)>	5.3	24.05	0.25
Completeness [%]	99.9	100	99.8
Redundancy	6.288	7.143	6.144
CC(1/2)	0.995	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES pH 7.5, 25% (wt/vol) PEG 3350