7KQG

Antibodies that engage the hemagglutinin receptor-binding site of influenza B viruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	40.130, 77.440, 225.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.600 - 2.600
R-factor	0.2285
Rwork	0.226
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqj
RMSD bond length	0.005
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.600	50.000	2.700
High resolution limit [Å]	2.600	30.000	2.600
Rmerge	0.172	0.021	0.828
Rmeas	0.184	0.022	0.885
Total number of observations	179861
Number of reflections	22576	17	2376
<I/σ(I)>	12.26	42.12	2.78
Completeness [%]	99.9	94.4	100
Redundancy	7.967	5.471	8.139
CC(1/2)	0.994	1.000	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM bis-Tris pH 6.5, 20% PEG MME 5000