7KQA

Crystal Structure of Glucosamine-6-phosphate deanimase from Strenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-07
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.920, 90.440, 126.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.520 - 1.650
R-factor	0.1591
Rwork	0.159
R-free	0.18110
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PARROT
Refinement software	PHENIX (1.19rc4-4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.049	0.025	0.374
Rmeas	0.050	0.025	0.414
Number of reflections	79758	1021	5156
<I/σ(I)>	38.59	112.86	3.82
Completeness [%]	98.7	99.3	87.4
Redundancy	17.001	25.558	5.31
CC(1/2)	1.000	1.000	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	287	StmaA.17926.a.B1.PW38775 [Barcode: 314016b7, PuckID: lhc5-4, Cryo: Direct, Concentration: 20 mg/mL] 170 mM Ammonium Acetate, 85 mM Sodium Citrate HCl pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol