7KNC

Crystal structure of the GH31 alpha-xylosidase (Xac1773) from Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.459000
Spacegroup name	P 21 21 2
Unit cell lengths	117.493, 146.632, 63.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.628 - 1.873
R-factor	0.1926
Rwork	0.191
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xvg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.628	1.970
High resolution limit [Å]	1.860	1.860
Number of reflections	89567	13272
<I/σ(I)>	17.65	6.66
Completeness [%]	98.3	91.2
Redundancy	9.7
CC(1/2)	0.998	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 mol/L potassium formate 20% PEG 3350