7KN8

Crystal structure of the GH74 xyloglucanase from Xanthomonas campestris (Xcc1752)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458550
Spacegroup name	P 21 21 21
Unit cell lengths	81.790, 100.430, 168.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.992 - 1.950
R-factor	0.2181
Rwork	0.216
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cn2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.992	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	99662	15178
<I/σ(I)>	11.02	2.5
Completeness [%]	98.1	93.7
Redundancy	12.191	10.12
CC(1/2)	0.997	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 mol/L sodium iodede 0.1 mol/L bis-tris propane 20% PEG 3350