7KN1

Crystal structure of UDP-glucose-4-epimerase (galE) from Stenotrophomonas maltophila with bound NAD and formylated UDP-arabinopyranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	72.270, 79.420, 153.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.080 - 1.450
R-factor	0.1596
Rwork	0.159
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1udc
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX (1.19rc-4022)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.080	43.080	1.490
High resolution limit [Å]	1.450	6.480	1.450
Rmerge	0.079	0.036	0.485
Rmeas	0.087	0.040	0.530
Total number of observations	917081
Number of reflections	156730	1933	11465
<I/σ(I)>	13.43	26.42	3.92
Completeness [%]	99.8	98.9	100
Redundancy	5.851	5.652	6.03
CC(1/2)	0.997	0.998	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	290	StmaA.00085.a.B1.PW38702 at 24.25 mg/ml was combined 1:1 (0.4 uL protein/precipitant) with 0.2 M Ammonium citrate dibasic, pH 5.0, and 20% (w/v) PEG 3350 and stored at 14C. No additional ligand was added for crystallization. Tray 312986f3; puck ocs5-3.