7KMU
Structure of WT Malaysian Banana Lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.526, 52.526, 221.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.790 - 1.510 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 4.100 | 1.510 |
| Rmerge | 0.060 | 0.035 | 0.414 |
| Rmeas | 0.062 | 0.036 | 0.433 |
| Rpim | 0.016 | 0.010 | 0.124 |
| Total number of observations | 699420 | ||
| Number of reflections | 49873 | 2818 | 2414 |
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.5 | 99.4 | 98.4 |
| Redundancy | 14 | 12.7 | 11.7 |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 1.6M Ammonium Sulfate, 2% Peg 1K, 0.1M HEPES 7.5 |
