7KMO

Crystal structure of the GH35 beta-galactosidase (Xac1772) from Xanthomonas citri in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458720
Spacegroup name	I 4
Unit cell lengths	116.680, 116.680, 97.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.447 - 1.753
R-factor	0.1958
Rwork	0.193
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d1i
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.447	1.850
High resolution limit [Å]	1.750	1.750
Number of reflections	63855	10477
<I/σ(I)>	8.92	0.64
Completeness [%]	97.4	99.8
Redundancy	6.47	5.958
CC(1/2)	0.998	0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	1.2 mol/L ammonium sulfate 8% glycerol 0.1 mmol/L tris