7KMN

Crystal structure of XAC1772, a GH35 xyloglucan-active beta-galactosidase from Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458730
Spacegroup name	I 4
Unit cell lengths	115.820, 115.820, 97.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.863 - 1.800
R-factor	0.1786
Rwork	0.177
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d1i
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.863	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	59344	9526
<I/σ(I)>	12.8	0.85
Completeness [%]	100.0	99.9
Redundancy	6.582
CC(1/2)	0.999	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 mol/L lithium sulfate 1.26 mol/L ammonium sulfate 0.1 mol/L tris