7KMJ

Hsa Siglec and Unique domains in complex with Sialyl Lewis C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	46.635, 58.173, 75.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.990 - 1.330
R-factor	0.1924
Rwork	0.191
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eq2
RMSD bond length	0.012
RMSD bond angle	1.510
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	1748709	2468
<I/σ(I)>	40.5
Completeness [%]	99.7
Redundancy	8
CC(1/2)	0.998	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	21.6 mg/ml in 20 mM Tris-HCl, pH 7.2. Equilibrate 1 ul protein and 2 ul reservoir solution over 50 ul of reservoir solution (0.1 M Succinate/Phosphate/Glycine pH 10.0 and 25% PEG 3350). Crystals were soaked in reservoir solution supplemented with 5 mM of sLec for 20 hr