7KM6
APOBEC3B antibody 5G7 Fv-clasp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.074, 68.823, 88.759 |
| Unit cell angles | 90.00, 107.05, 90.00 |
Refinement procedure
| Resolution | 84.860 - 1.670 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xcq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 84.860 | 84.860 | 1.680 |
| High resolution limit [Å] | 1.650 | 9.050 | 1.650 |
| Rmerge | 0.088 | 0.050 | 0.833 |
| Rmeas | 0.104 | 0.059 | 0.998 |
| Rpim | 0.053 | 0.031 | 0.537 |
| Total number of observations | 1657 | 7310 | |
| Number of reflections | 71357 | 479 | 2724 |
| <I/σ(I)> | 7.6 | 20.8 | 0.9 |
| Completeness [%] | 97.5 | 99 | 74.1 |
| Redundancy | 3.7 | 3.5 | 2.7 |
| CC(1/2) | 0.996 | 0.997 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM Bis-Tris/Hydrochloric acid pH 6.5, 400 mM sodium chloride, 30% (w/v) PEG 3350 |
