7KL7

Helicobacter pylori Xanthine-Guanine-Hypoxanthine Phosphoribosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-05
Detector	BRUKER SMART 6000
Wavelength(s)	0.95365
Spacegroup name	P 1 21 1
Unit cell lengths	40.201, 51.544, 82.687
Unit cell angles	90.00, 96.09, 90.00

Refinement procedure

Resolution	34.540 - 1.470
R-factor	0.173
Rwork	0.172
R-free	0.19430
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	1.722
Data reduction software	ARP/wARP
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.670	11.490
High resolution limit [Å]	1.470	1.470
Number of reflections	57196	256
<I/σ(I)>	10.6
Completeness [%]	99.0
Redundancy	9
CC(1/2)	0.800	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.1 M Bis-Tris propane, pH 5.5, 0.2 M ammonium sulfate, 25% PEG3350