7KJO

crystal structure of PLEKHA7 PH domain biding SO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 41
Unit cell lengths	64.830, 64.830, 59.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.842 - 1.450
R-factor	0.1539
Rwork	0.153
R-free	0.17140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1upr
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.842	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.050	0.362
Number of reflections	46929	4283
<I/σ(I)>	22.2
Completeness [%]	99.2
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	protein in 180 mM NaCl and 20 mM sodium phosphate mixed with 25% glycerol, 2 M ammonium sulfate