7KJ7

Plasmodium falciparum protein Pf12p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953647
Spacegroup name	P 21 21 21
Unit cell lengths	51.999, 74.175, 183.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.257 - 2.800
R-factor	0.2526
Rwork	0.250
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ymo
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.257	47.257	2.960
High resolution limit [Å]	2.790	8.270	2.790
Rmerge	0.183	0.057	1.484
Rmeas	0.197	0.062
Total number of observations	134842
Number of reflections	18315	791	2880
<I/σ(I)>	8.44	26.94	1.14
Completeness [%]	99.7	99	98.8
Redundancy	7.362	6.526	7.208
CC(1/2)	0.995	0.996	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	2.5% MPD, 0.02 M alanine, 0.1 M bicine-tris pH 8.5, 0.02 M glycine, 0.02 M lysine, 12.5% PEG1000, 12.5% PEG3350, 0.02 M serine, 0.02M sodium glutamate, 0.2 M magnesium chloride