7KIQ

Crystal structure of the mouse lipin-2 M-Lip domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	70.706, 84.612, 97.511
Unit cell angles	90.00, 108.90, 90.00

Refinement procedure

Resolution	47.353 - 2.523
R-factor	0.2142
Rwork	0.212
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kih
Data reduction software	DIALS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.650	2.600
High resolution limit [Å]	2.523	2.530
Rmerge	0.256	2.124
Rmeas	0.277	2.291
Rpim	0.105	0.856
Total number of observations		18637
Number of reflections	36291	2634
<I/σ(I)>	5.5	1
Completeness [%]	99.3	98.9
Redundancy	6.9	7.1
CC(1/2)	0.988	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	PEG 3350, CaAcetate