7KIQ
Crystal structure of the mouse lipin-2 M-Lip domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.706, 84.612, 97.511 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 47.353 - 2.523 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kih |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.650 | 2.600 |
| High resolution limit [Å] | 2.523 | 2.530 |
| Rmerge | 0.256 | 2.124 |
| Rmeas | 0.277 | 2.291 |
| Rpim | 0.105 | 0.856 |
| Total number of observations | 18637 | |
| Number of reflections | 36291 | 2634 |
| <I/σ(I)> | 5.5 | 1 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 0.988 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | PEG 3350, CaAcetate |
