7KIL

Crystal structure of the mouse lipin-1 M-Lip domain with zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.282
Spacegroup name	P 1 21 1
Unit cell lengths	34.284, 58.554, 42.897
Unit cell angles	90.00, 100.06, 90.00

Refinement procedure

Resolution	42.238 - 1.898
R-factor	0.2255
Rwork	0.222
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kih
Data reduction software	xia2
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.238	42.230	1.950
High resolution limit [Å]	1.898	8.500	1.898
Rmerge	0.284	0.189	1.190
Rmeas	0.308	0.206	1.309
Rpim	0.118	0.081	0.536
Total number of observations		1012	5694
Number of reflections	13277	155	966
<I/σ(I)>	3.9	8.6	1.2
Completeness [%]	100.0	99.6	100
Redundancy	6.6	6.5	5.9
CC(1/2)	0.971	0.957	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	ZnAcetate