7KIH

Crystal structure of the mouse lipin-1 M-Lip domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	193
Detector technology	CCD
Collection date	2017-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	44.139, 59.319, 67.442
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.411 - 1.467
R-factor	0.1837
Rwork	0.182
R-free	0.21560
Structure solution method	SAD
Data reduction software	pointless
Data scaling software	Aimless (0.5.23)
Phasing software	autoPROC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.440	67.440	1.490
High resolution limit [Å]	1.467	8.040	1.467
Rmerge	0.046	0.043	0.590
Rmeas	0.051	0.049	0.706
Rpim	0.021	0.022	0.379
Total number of observations		584	1710
Number of reflections	15308	121	609
<I/σ(I)>	14.8	36	1.1
Completeness [%]	98.7	99.2	81.7
Redundancy	5.2	4.8	2.8
CC(1/2)	0.998	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	PEG 3000, Tris HCl