7KHX
Mouse GITR-GITRL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.540, 64.820, 81.230 |
| Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
| Resolution | 40.887 - 3.206 |
| R-factor | 0.245402523018 |
| Rwork | 0.244 |
| R-free | 0.27768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.823 | 3.320 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 9139 | 4001 |
| <I/σ(I)> | 6.61 | |
| Completeness [%] | 90.6 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.995 | 0.028 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 17% PEG1500 and 0.1 M Bicine pH 8.0 |
