7KH2
Structure of N-citrylornithine decarboxylase bound with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793217 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.839, 279.061, 108.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.650 - 2.050 |
| R-factor | 0.1741 |
| Rwork | 0.174 |
| R-free | 0.19920 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.661 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.021 | 0.322 |
| Number of reflections | 237296 | 6761 |
| <I/σ(I)> | 35.95 | 2.41 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 13.2 | 12.2 |
| CC(1/2) | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.7 M Ammonium sulfate, 0.085 M Hepes pH 7.5, 1.7% PEG400, 15% Glycerol, and 10 mM DTT |
