7KGC
Crystal structure of a perchloric acid-soluble protein (PSP) from Trichomonas vaginalis at 1.95 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.950, 81.950, 129.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.684 - 1.950 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qah |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.082 | 0.440 |
| Number of reflections | 35775 | 2529 |
| <I/σ(I)> | 14.1 | 2.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.990 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride, pH 8.5 and 30% polyethylene glycol 400 |
