7KGA

Crystal structure of a mosquito-borne flavivirus dumbbell RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-17
Detector	RDI CMOS_8M
Wavelength(s)	1.10208
Spacegroup name	P 1 21 1
Unit cell lengths	65.930, 49.670, 73.460
Unit cell angles	90.00, 99.74, 90.00

Refinement procedure

Resolution	44.740 - 2.100
R-factor	0.2298
Rwork	0.228
R-free	0.25850
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (Phenixdev-3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.740	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.130	0.878
Rmeas	0.137	0.926
Rpim	0.041	0.292
Number of reflections	27633	2741
<I/σ(I)>	17.95	3.17
Completeness [%]	99.8	99.89
Redundancy	10.9	10
CC(1/2)	0.998	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM Bis-Tris pH 7.0, 25 mM NaCl, 25 mM LiCl, 4.5 mM CaCl2, 50 mM Guanidine HCl, 23.75% polyethylene glycol 2000