7KFL

Crystal structure of the cargo-binding domain from the plant class XI myosin (MyoXIk)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	147.062, 54.333, 113.432
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	47.360 - 2.350
R-factor	0.2343
Rwork	0.232
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j5l
RMSD bond length	0.002
RMSD bond angle	1.145
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.126	1.368
Number of reflections	37294	3671
<I/σ(I)>	7.08
Completeness [%]	99.3
Redundancy	3.2
CC(1/2)	0.996	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	75 mM Bis-Tris pH 7.5, 0.2 M MgCl2, 25% (w/v) PEG 3350 and 1 mM TCEP