7KET
Factor H enhancing human antibody fragment (Fab) to meningococcal Factor H binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.580, 86.030, 107.890 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 107.110 - 2.000 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model constructed from human Fab; FHbp ID 1 (3KVD) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | Coot (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.110 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.101 | 1.660 |
| Rmeas | 0.123 | 2.090 |
| Rpim | 0.071 | 1.240 |
| Number of reflections | 46166 | 3877 |
| <I/σ(I)> | 8.02 | |
| Completeness [%] | 95.0 | 82.44 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | HEPES, polyethylene glycol 3350, tryptone, sodium azide |
