7KE3

Heavy chain ferritin with C-terminal EBNA1 epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 42 21 2
Unit cell lengths	217.882, 217.882, 147.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.720 - 2.200
R-factor	0.2422
Rwork	0.241
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4a
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.720	48.720	2.240
High resolution limit [Å]	2.200	12.050	2.200
Rmerge	0.336	0.056	2.549
Rmeas	0.342	0.057	2.597
Rpim	0.065	0.012	0.493
Total number of observations	4871537	31305	240131
Number of reflections	177978	1241	8703
<I/σ(I)>	8.9	29.8	1.6
Completeness [%]	99.9	98.6	99.8
Redundancy	27.4	25.2	27.6
CC(1/2)	0.999	1.000	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1M HEPES pH 7.0, 30% Jeffamine M-600 pH 7.0