7KD9

Crystal Structure of Gallic Acid Decarboxylase from Arxula adeninivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	91.632, 265.301, 93.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.610 - 1.940
R-factor	0.1933
Rwork	0.191
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jmr
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.940	1.940
Number of reflections	168891	16310
<I/σ(I)>	15.2
Completeness [%]	99.5	96.8
Redundancy	4.9	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	PEG 3350, KSCN, MES