7KCI
DETERMINANTS OF REPRESSOR/OPERATOR RECOGNITION FROM THE STRUCTURE OF THE TRP OPERATOR BINDING SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Wavelength(s) | 1.5400 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 32.900, 32.900, 95.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.950 |
| R-factor | 0.17 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | standard B-DNA model |
| Phasing software | ULTIMA |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 4034 |
| Redundancy | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | PEG 200, NA CACODYLATE, SPERMINE_HCL, MGCL2 |
