7KCG

Salivary protein from Culex quiquefasciatus that belongs to the Cysteine and Tryptophan-Rich (CWRC) family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	34.800, 59.680, 85.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.740 - 1.870
R-factor	0.2308
Rwork	0.229
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology
RMSD bond length	0.009
RMSD bond angle	1.360
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.740	34.740	1.920
High resolution limit [Å]	1.870	8.360	1.870
Rmerge	0.038	0.029	0.367
Rmeas	0.041	0.032	0.410
Total number of observations	113172
Number of reflections	15256	188	1079
<I/σ(I)>	23.77	38.87	3.48
Completeness [%]	99.6	91.7	96
Redundancy	7.418	5.367	4.848
CC(1/2)	0.999	0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	4M Sodium formate (Condition 33, Crystal Screen, Hampton Research)