7KC7

Biotin Carboxylase domain of Thermophilic 2-Oxoglutarate Carboxylase bound to ADP without Magnesium with disordered phosphate tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.968, 120.489, 124.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.170 - 2.200
R-factor	0.1773
Rwork	0.175
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ULZ
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	50075	2489
<I/σ(I)>	26.54
Completeness [%]	99.6	100
Redundancy	6.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	PEG 3350, 0.02M Citric Acid, 0.08M Bis-Tris-Propane