7KBO

Reverse Transcriptase Diabody with S82bC, R83T Mutations Crystallized in C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	73.707, 86.018, 36.682
Unit cell angles	90.00, 117.98, 90.00

Refinement procedure

Resolution	33.730 - 2.200
R-factor	0.18
Rwork	0.178
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vrp
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (v1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.107
Rmeas	0.047	0.128
Number of reflections	10076	493
<I/σ(I)>	27.8
Completeness [%]	97.7	96.3
Redundancy	3.4
CC(1/2)	0.992	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	6% v/v Propanol, 0.1 M sodium acetate trihydrate pH 4.5, 26 % PEG 550 MME