7KB2
Putative ankyrin repeat domain-containing protein from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.587, 56.354, 106.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.820 - 1.600 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22560 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.127 | 0.097 | 1.071 |
| Rmeas | 0.134 | 0.102 | 1.174 |
| Rpim | 0.040 | 0.030 | 0.473 |
| Number of reflections | 41876 | 2303 | 2036 |
| <I/σ(I)> | 8.1 | 1.75 | |
| Completeness [%] | 99.4 | 100 | 99.4 |
| Redundancy | 8.2 | 11.1 | 5.9 |
| CC(1/2) | 0.995 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M sodium acetate, 0.1 M MES:NaOH, 30% PEG 400 |
