7K96
Human DNA polymerase beta ternary complex with templating cytosine and incoming deoxyguanosine diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.800, 80.400, 55.500 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.1892 |
| Rwork | 0.183 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.109 | 0.902 |
| Rpim | 0.048 | 0.525 |
| Number of reflections | 23635 | 1166 |
| <I/σ(I)> | 11.4 | 1.3 |
| Completeness [%] | 99.1 | 97.7 |
| Redundancy | 4.5 | 2.4 |
| CC(1/2) | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50 mM imidazole, 350 mM sodium chloride, 16-17% PEG3350 |
