7K81
KIR3DL1*005 in complex with HLA-A*24:02 presenting the RYPLTFGW peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.807, 61.385, 66.989 |
| Unit cell angles | 94.32, 99.82, 105.97 |
Refinement procedure
| Resolution | 34.020 - 2.000 |
| R-factor | 0.1928 |
| Rwork | 0.190 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vh8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.020 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 46315 | 25532 |
| <I/σ(I)> | 8.4 | 2.9 |
| Completeness [%] | 96.0 | 95 |
| Redundancy | 3.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 14% PEG 3350, 2% tacsimate, pH 5.0, and 0.1 M trisodium citrate, pH 5.6 |
