7K7W

The X-ray crystal structure of SSR4, an S. pombe chromatin remodelling protein: native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.911684
Spacegroup name	P 21 21 21
Unit cell lengths	50.096, 67.506, 67.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 1.770
Rwork	0.177
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7k7v
RMSD bond length	0.012
RMSD bond angle	1.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	1.810
High resolution limit [Å]	1.770	1.770
Rmerge	0.169
Rpim	0.047	0.893
Number of reflections	23002	1297
<I/σ(I)>	10.9	1.1
Completeness [%]	100.0	100
Redundancy	13.5	8
CC(1/2)	0.998	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	Crystallisation experiments were set up in SD2 sitting drop plates at 8 C with 200 nL protein plus 200 nL reservoir with 50 uL of reservoir in the wells. The protein concentration was 5 mg/mL. Reservoir conditions contained 1.5 to 1.9 M ammonium sulfate, 0.7-12% dioxane and either 100 mM MES, 100 mM bis-tris or 10% (v/v) malate-MES-tris buffer at a pH between 5.5 and 5.8