7K7U
BetaB2-crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 78.655, 113.192, 340.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.030 |
| R-factor | 0.19202 |
| Rwork | 0.189 |
| R-free | 0.25037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ytq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.719 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 3.110 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 1.800 | |
| Rpim | 0.771 | |
| Number of reflections | 59687 | 4370 |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 6.3 | 6.3 |
| CC(1/2) | 0.992 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | 0.1 M HEPES pH 7.5, 10% w/v PEG 8,000, 8% (v/v) ethylene glycol |
