7K7T
Crystal structure of human MORC4 ATPase-CW in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-06-14 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.2782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.424, 109.934, 70.390 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 47.110 - 2.940 |
| R-factor | 0.2397 |
| Rwork | 0.237 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6o1e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rpim | 0.079 | 0.285 |
| Number of reflections | 16000 | 1280 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 94.7 | 75.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Hepes 7.5, 20% Jeffamine ED-2003 |
