7K76

Crystal structure of MAD2-6 IgG Fab in complex with PfCSP N-terminal peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03316
Spacegroup name	P 21 21 21
Unit cell lengths	69.390, 77.320, 181.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.113 - 2.140
R-factor	0.1854
Rwork	0.184
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model
RMSD bond length	0.002
RMSD bond angle	0.581
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.320	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.119	0.522
Rpim	0.050	0.216
Number of reflections	54785	4429
<I/σ(I)>	10	3.5
Completeness [%]	100.0	100
Redundancy	6.6	6.8
CC(1/2)	0.996	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.4 M NH4-sulfate, 0.1 M MES pH 6.0