7K75

Crystal structure of MAD2-6 IgA Fab in complex with PfCSP N-terminal peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 21
Unit cell lengths	73.136, 153.680, 193.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.960 - 2.500
R-factor	0.1945
Rwork	0.192
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.123	0.789
Rpim	0.060	0.494
Number of reflections	75359	3252
<I/σ(I)>	10.3	0.8
Completeness [%]	99.0	87.1
Redundancy	5	2.9
CC(1/2)	0.905	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	40% PEG-300, 0.1M phosphate-citrate pH 4.2, final pH 5.2